PhD offer in Nonlinear Optical Microscopy

Aller au contenu principal Aller au menu Aller à la recherche

Accessibilité

Intranet

Annuaire

Toggle navigation

L’Institut d’Optique

Mission, Institution

Gouvernance

Organigramme

Faits et chiffres

Soutenir l’Institut d’Optique

Histoire

Sciences et technologies de la lumière

Campus national

Bordeaux

Paris-Saclay

Saint-Étienne

Mediathèque

Dépôt légal des thèses

Publications des laboratoires

Recrutement

Brochures

Accès

Formations

Cycle ingénieur

Déroulé de la formation

Enseignement Expérimental

Langues et cultures

International

Stages

3 filières

Doubles diplômes & Masters

Accords et partenariats internationaux

L'Institut d'Optique et Erasmus+ 2021-2027

Débouchés

Démarche qualité

Validation des Acquis de l'Expérience (VAE)

FAQ

Doctorat

Inscription et ré-inscription en thèse au Laboratoire Charles Fabry

Écoles doctorales

Recherche

Laboratoire Charles Fabry (LCF)

Laboratoire Photonique, Numérique et Nanosciences (LP2N)

Laboratoire Hubert Curien

Publications de la recherche à l'Institut d'Optique

Soutenances de thèse Institut d'Optique Graduate School

Projets de recherche européens et internationaux

Entreprises et Innovation

Les campus d'innovation 503

L'apprentissage à SupOptique

Recruter un·e apprenti·e

Forum de recrutement

Pourquoi et comment devenir apprenti·e

Témoignages d’apprenti•e•s

Comment rejoindre l'apprentissage

Être apprenti·e ca implique quoi ?

FAQ apprenti·es

Aides financières pour les apprenti·es

Mobilité internationale des apprenti·es

Indicateurs / Qualité

Accompagnement handicap

Livret d'apprentissage numérique

Partenariat École

Devenir membre du Partenariat École

Les membres du Partenariat École

Offres de stage, d’alternance et d’emploi

Formation continue

Taxe d'apprentissage

Déposer une offre de stage

Campus

Vie associative

Vie sur les 3 campus

Vie étudiante

Egalité de genres

Diversité-Handicap

Actions en collaboration Etudiant·es / Enseignant·es

Aides aux étudiant.es

Mobilités à l'international

Admission

Inscription

Recrutement par voie universitaire

Recrutement après une classe préparatoire

Étudiants internationaux

Oraux : infos pratiques

PhD offer in Nonlinear Optical Microscopy

Fil d'Ariane

Accueil

Offres d’emploi

PhD offer in Nonlinear Optical Microscopy

Recherche

Thèse et post-doc

Établissement Talence

Imagerie innovante et biologie quantitative

×

Envoyer cette page à un ami

Photonics Numerical and Nanosciences Laboratory (LP2N) - https://www.lp2n.institutoptique.fr/ IOGS - CNRS - University of Bordeaux,

1 rue François Mitterrand, 33400 Talence

Context

The mechanical properties of biological systems play a complex role in determining the physiological functions of tissues and appear to be dominant factors in many processes of development, homeostasis and pathology. Interestingly, tissues with properties as different as bone, skin or tendon are mainly composed of the same elementary units. Hence, their specific mechanical properties are directly linked to their sub-microscopic organisation. Nowadays, understanding the structure/function link in connective tissues faces the challenge of probing the multiple scales involved in constructing macroscopic properties from individual structures in highly complex samples. Indeed, standard techniques remains either invasive, limited to the surface, or not sufficiently resolving. The advent of multiphoton microscopy, based on the nonlinear interaction between laser pulses and the constituents of biological samples, has revolutionised the way we observe living organisms. Notably, Second Harmonic Generation (SHG) microscopy has recently emerged as the gold standard for collagen imaging in thick samples, enabling label-free visualization of fibrillar distribution with high intrinsic specificity and sub-micron resolution.

Objective

In this context, the aim of the research project is to implement a multimodal imaging platform, compatible with simultaneous mechanical assay, to probe the multiscale morpho-mechanical properties of connective tissues. The first step, which is the objective of this Master project, is to set up a state-of-the art SHG microscope. Upon operational, this instrument will be validated on mouse vocal folds by probing and quantifying collagen architecture in the different layers, from epithelium to the vocalis muscle, through the lamina propria. As a proof-of-concept, monitoring fine changes after laser-induced lesions will demonstrate the potential of this method to characterize local alteration of the tissue at microscopic scale.

Environment -

This project will take place in the Nano-BioMicroscopy team at LP2N (Institut d’Optique d’Aquitaine). The team works at the crossroads between nanoscience, optics and bio-imaging to design and study innovative nanostructures and to investigate complex biological system at the nanoscale. In particular, the group has a well-known expertise in infrared imaging, super-resolution microscopy and single particle tracking.

Candidate -

This project is largely experimental and involves aspects of nonlinear microscopy (femtosecond laser alignment, image acquisition), data analysis (ImageJ /Python/ Matlab) and basic tissue preparation. We are seeking for candidate with a background in physics/experimental optics and a strong motivation to work in an interdisciplinary environment. Notion in microscopy, programming (Python) and image/signal processing would be an asset.

To apply, please send a CV, a motivation letter, your transcripts, and at least one reference letter to Stéphane Bancelin (). Possibility to continue the project during a PhD with a secured ANR funding (project ‘’HaBIm’’) to implement and validate the complementary imaging modalities (Brillouin and Raman).

L’Institut d’Optique

Mission, Institution

Soutenir l’Institut d’Optique

Histoire

Sciences et technologies de la lumière

Campus national

Mediathèque

Recrutement

Brochures

Accès

Formations

Cycle ingénieur

Doctorat

Recherche

Laboratoire Charles Fabry (LCF)

Laboratoire Photonique, Numérique et Nanosciences (LP2N)

Laboratoire Hubert Curien

Publications de la recherche à l'Institut d'Optique

Soutenances de thèse Institut d'Optique Graduate School

Projets de recherche européens et internationaux

Entreprises et Innovation

Les campus d'innovation 503

L'apprentissage à SupOptique

Partenariat École

Formation continue

Taxe d'apprentissage

Déposer une offre de stage

Campus

Vie associative

Vie sur les 3 campus

Mobilités à l'international

Annuaire de l'institut

Mentions legales

Plan de site

Site réalisé par Intuitiv Intecartive